Spectral Analyzers

Cyteck Webinar Spectral Cytometry

• Video #1 (Skip first 10 mins)  (May 14,2020)
  • Introduction to Spectral Cytometry using Cytek® Aurora #1(May 11, 2020)
  • Exercise:
    • 5L Spectrum Overlay Exercise
    • Aurora 5L Detector Array Configuration
    • Exercise 1 – 5 Laser
    • N9-20017 Rev A 5L UVVBYGR Fluor Guide

• Video #2 (May 14,2020)
  • Introduction to Spectral Cytometry using Cytek® Aurora #2 (May 12, 2020)
  • Exercise:
    • Exercise 2

• Video #3 (May 14,2020)
  • Introduction to Spectral Cytometry using Cytek® Aurora #3 (May 13, 2020)
  • Exercise:
    • EXERCISE 3
    • Exercise 3 supplemental materials_ww training
    • Exercise 3 – submissions + spread question
    • 32C CSI Template – BatchAnalysis
    • 32C CSI Template Instructions vFinal
    • Fluorencent Signal-Dye and Protein is it a tandem
    • NK panel_fluor characterization

• Video #4 (May 14,2020)
  • Introduction  to Spectral Cytometry using Cyteck Aurora #4 (May 14,2020)
  • Anitobody Titration_Roederer

• Video #5 (May 18,2020)
  • Introduction to Spectral Cytometry using Cyteck Aurora # 5 (May 18, 2020)
  • N9_20037_Rev._A_SpectroFlo_Video_Tutorials_Guide

System startup and shutdown

• Startup and shutdown Guide
• System startup & QC video
• System shutdown video
• Aurora Software Quick Reference Guide

Training and Troubleshooting Guide

• For training contact James Marvin
• Create and unmix an experiment
• Reference Control QC Guide
• Video Tutorials Guide
• Aurora User Training Presentation UofChicago
• The Importance of Titrating Viability Dyes on the Aurora Spectral Cytometer (May 13, 2020)

Panel Design

• 5Laser Fluorchrome Selection Guide
• Panel design matrix
• 5L Spectrum Overlay Exercise
• Fluorencent Signal-Dye and Protein is it a tandem

Spectra viewer

• Aurora Spectral Viewer (Cytek)
• Aurora Spectra-analyzer (BioLegend)

Extra links

• Aurora Resources
• cytekbio video-tutorial
• Introduction to full Spectrum Cytometry
• Panel Design and Optimization for High‐Dimensional Immunophenotyping Assays Using Spectral Flow Cytometry (May 13, 2020)
• High‐Dimensional Fluorescence Cytometry (May 13, 2020)