This collection of Flow Cytometry resources is designed to facilitate learning from home during the Covid-19 pandemic. We will continue to update resources and materials so check back regularly.
Flow basics
- Flow Cytometry Animation
- (Invitrogen) Introduction to Flow Cytometry
- The Principle of Flow Cytometry and FACS (1- Flow Cytometry)
- MIFlowCyt: The Minimum Information about a Flow Cytometry Experiment
Cyto University
- CYTO University Online Learning by CYTOU – CYTO University
- (Cyto Seminars) CYTO University Online Learning by CYTOU | CYTO University
- Step-by-Step Multi-parameter Panel Design – CYTO University
- Single Cell Analysis of Autofluorescence Lifetime Images
- Cytometry Part A Spotlight: Rapid Comparative Immunophenotyping of Human Mesenchymal Stromal Cells by a Modified Fluorescent Cell Barcoding Flow Cytometric Assay
- Flow Cytometric Analysis of Endothelial Colony Forming Cells and Hematopoietic Progenitor Cells in Lung Vascular Disease by Kewal Asosingh and Imaging Flow Cytometry in the Study of Immune Cell Functions by Andrew Filby
Vendor Training
- BD Biosciences Flow Training
- BD Biosciences Intracellular Training
- ThermoFischer Flow Cytometry Training
Cytometry Part A
- Cytometry Part A
- SARS‐CoV‐2, the Virus that Causes COVID‐19: Cytometry and the New Challenge for Global Health
- SYTO probes in the cytometry of tumor cell death
- FlowSOM: Using self‐organizing maps for visualization and interpretation of cytometry data
- Flow cytometry controls, instrument setup, and the determination of positivity
- From “truly naive” to “exhausted senescent” T cells: When markers predict functionality
- OMIP-063: 28-Color Flow Cytometry Panel for Broad Human Immunophenotyping
Compensation/unmixing
- Compensation in multicolor flow cytometry – Szalóki – 2015 – Cytometry Part A
- Compensation in Flow Cytometry – Roederer – 2002
- Authors response to correspondence about an improved compensation method
- Generalized unmixing model for multispectral flow cytometry utilizing nonsquare compensation matrices – Novo – 2013 – Cytometry Part A
- (Bio-Rad) Fluorescent Compensation – Flow Cytometry Guide
- (BD) An Introduction to Compensation for Multicolor Assays on Digital Flow Cytometers
- (FlowJo) Part 1 Compensation, Part 1
- (FlowJo) Part 2 Compensation, Part 2
Full Spectrum Flow Cytometry
- Link to aurora resources
- Applying Panel Design Principles to Investigate COVID-19 Immune Response with the Cytek Aurora (May 10)
- DeNovo Spectral Flow Cytometry and Data Analysis Techniques
- Spectral Flow Cytometry
- (ThermoFisher) Spectral Flow Cytometry Fundamentals
- Taking a Closer Look at Spectral Flow Cytometry – Andor Learning Centre
Quarantine Webinars
- Introduction to NovaFlours
- 200416 Gardner Full Spectrum Cytometry
- BD Webinars (May 10,2020)
- Getting Started with Spectral Cytometry Using Cytek Aurora (May 10, 2020)
- Analyzing High-Resolution Single Cell Gene Expression Data (May 10, 2020)
Panel Design Webinars
- Stanford: Hands On-Panel Design (May 19, 2020)
- BD Panel design Webinar (May 19, 2020)
- BioRad Webinar: Multicolor panel building in flow cytometry (May 19, 2020)
- Not Your Typical Flow Cytometry Panel Design Webinar: A tool kit for selecting antibodies with the complete panel in mind (May 19, 2020)
FlowJo (videos)
- FlowJo Media
- Concatenation and tSNE Webinar
- Dimensionality Reduction with the tSNE Algorithm (5/12/2020)
- Concatenation, tSNE, and Clustering with Tim Quinn Crawford, PhD 5/17/18
- Intro to FlowJo v10 with Jack 9.7.17
Computational Resources
- High Parameter Flow Cytometry and a New Computational Platform Reveal Unique Cell Phenotypes that Predict Melanoma Outcomes
- Introduction to R – CYTO University – Online Learning by CYTOU
- Dimensionality reduction for visualizing single-cell data using UMAP Becht Nat Biotech 2018 (5/12/2020)
- Why UMAP is Superior over tSNE (5/12/2020)
- Comprehensive Guide on t-SNE algorithm with implementation in R & Python (5/12/2020)
- A Basic Overview of Using t-SNE to Analyze Flow Cytometry Data (5/12/2020)
UCFlow Blogspot
Cytometry Resources Blog