Frequently Asked Questions2021-04-06T18:35:04+00:00
Q1. How can I start using your facility?2021-10-13T15:19:09+00:00

In order to get access to the scheduler to reserve instruments, you will need to fill out the Work Authorization Form.

Analyzer Training- We normally like to do instrument training 1 on 1 with actual samples. That way we can tailor the training specific to your assay and set up a template and instrument settings with you. We highly recommend the educational seminars prior to training.

 

Q2.How can I get access to facility instruments after hours?2020-11-04T15:45:33+00:00

Once you are fully trained on a particular instrument you are eligible for after hours access. In order to get your ID card activated for WINTROBE you need to go to the core facilities administration office located at SOM 5C124. Terra Curley (3-2926) or Audrey Grisham (3-2928) will need your ID number and also the 6 numbers following the 2* on the back of the card. If you need access to HCI contact Gayle Kartchner at gayle.kartchner@hci.utah.edu .If you need access to SMBB email James Marvin at jmarving@cores.utah.edu.

Q3. How can I get involved with the Flowjo Site License?2021-10-14T16:00:42+00:00

I will try and make this brief but there are a lot of details that need explaining. Currently, once a year Flowjo sends me a bill at the end of the billing cycle. This bill shows how many quarters a particular computer was registered with the Utah site license. If you deleted your computer or signed up half way through the year you were only billed a partial amount. Moving forward, billing will happen at the beginning of the year and registrations and authentication of the software will be handled through creating and logging into a “Flowjo Portal” account. I will be sending you an invite through my administrative tools to create your account shortly. IT IS YOUR RESPONSIBILITY TO ACTIVATED AND DELETE COMPUTERS WHEN YOU MOVE LABS OR STOP USING FLOWJO. IF YOU DONT, YOUR PI WILL GET BILLED. 

The one really nice feature is that you can have multiple computers or “authorized devices” tied to your account. So you can have an office computer, laptop and home computer all registered but only pay for the equivalent of one computer. I have a feeling this might save a few labs some money. If you are a lab that doesn’t do a lot of flow you can also have a “shared computer”. Everyone in the lab would have to create a portal account to log into the shared computer but your lab would only pay for one computer. (If you think  your lab would like a shared computer, please let me know).

This is going to be a great time to purge all the computers that are getting billed but are not actually getting utilized.

So here is what needs to happen.

  1. Create Flowjo Portal account. I will send you and invite. If you don’t get an invite let me know.
  2. Sign-in to your FlowJo Portal account through my invite and accept the group license invitation by clicking the blue banner/link.
  3. Open FlowJo 10.5.2 or higher and follow the attached PDF instructions for FlowJo sign-in. *Delete your serial number from the serial number field, within the FlowJo license screen before signing in with your username to avoid license confusion.
  4. After you sign-in to FlowJo with your FlowJo Portal username/password, FlowJo will communicate your computers HWA to your FlowJo Portal account. You can manage the computers that your account allows access to via your FlowJo Portal account/Authorized Devices. Each user will be able to access FlowJo on up to 4 computers of their choosing annually one computer at a time.
  5. Enjoy Flowjo

 

Shared Computers can be used by multiple users, one at a time. The computer holds the license, so any users with a FlowJo Portal account (paid or free) can access FlowJo on said Shared Computer.

https://docs.flowjo.com/portal/shared-devices/

Authorized Devices (computers) are for one account. Meaning each user, who has a user seat assigned to them, will be able to access FlowJo on up to 4 devices of their choosing, one device at a time.

https://docs.flowjo.com/portal/authorized-devices/

Q4. Which cell sorter should I use.2020-11-04T17:04:16+00:00
Q5. What do I need to bring to sort cells?2020-11-04T17:07:08+00:00

1. Samples- Your samples can be in 5ml tubes or 15ml conical. You should aim for a concentration around 10 million/ml for cell lines or 15 million/ml for lymphocytes. If you don’t have nearly that many cells, don’t resuspend in less than 500ul. Buffer can be anything that the cells are happy in. More detailed info here

2. Controls- For fluorescent protein work we need an untransfected sample to set up the instrument. For multicolor experiments we need unstained in addition to single color controls.

3. Collection Tubes- We can collect into eppendorf, 5ml, 15ml 50ml or 96well plates. Each collection tube should have collection media already in the tube.

4. Extra media- In case there is a clog and a sample needs to be resorted.

5. Jump Drive- If you need your data files

6. Ice- If appropriate.

Q6. How should I acknowledge the facility in my publication?2020-11-23T20:31:36+00:00

The most important acknowledgement for the facility and university is referencing the shared instrumentation grant for the Imagestream for any work related to your publication. Something like this would work.

“Research reported in this publication was supported by the Office Of The Director of the National Institutes of Health under Award Number S10OD026959”

Also referencing the NCI Award Number 5P30CA042014-24 is helpful. 

THANKS!!!!

Q7. Whats the deal with this 96well single cell sorting business?2020-11-04T17:08:08+00:00

That is correct, we can sort single cells into a variety of 96 well plates, and also 384 well plates. This can be accomplished on the 5 laser Aria or the 4 laser Aria. The setup where we target the wells takes about 5-10 min and then it takes about 90 seconds  to run a plate. A single cell will come out in about 2.3nl (very tiny droplets!!) so you definitely want to have media already in your plates. And there is generally no need to spin and wash when you are done.

Q8. Who are our current Sales Reps and Discount Codes.2020-11-04T17:08:39+00:00

Tonbo- Evan Cardani evan.cardani@tonbobiotech.com
Thermofisher- Jeff Sun jeff.sun@thermofisher.com
BD- Alicia Brittain- Alicia.Brittain@bd.com
Biolegend- Danielle Duncan dduncan@biolegend.com  or Avi Perna aperna@biolegend.com

Q9. Are the University of Utah instruments available on Fluorofinder2020-11-04T17:09:03+00:00
Q10. Where are your instruments located?2020-11-04T17:10:16+00:00

We have instruments all over campus. Here is a map to help you find the closest to your lab.

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