-There are now 3 Beckman Coulter Cytoflex instruments on campus. These instruments have different detectors, electronics, software than what most people are used to, i.e., BD and FacsDiva.
-Flowjo default settings are generally set up to analyze data collected on BD instruments.
-Getting data from the Cytoflex instruments to be visualized properly may take some adjustments to settings you may not normally use.
-Presentation above has some Treg data that highlights the issues, and ways of correcting the visualization